Bacillus Subtilis

Bacillus subtilis, known also as the hay bacillus, is a Gram-positive bacterium, found in soil. B. subtilis is rod-shaped, and can form a tough, protective endospore, allowing it to tolerate extreme environmental conditions. B. subtilis has historically been classified as an obligate aerobe, though evidence exists that it is a facultative aerobe. B. subtilis is considered the best studied Gram-positive bacterium and a model organism to study bacterial chromosome replication and cell differentiation. It is one of the bacterial champions in secreted enzyme production and used on an industrial scale by biotechnology companies.


B.subtilis bacteria produce a class of lipopeptide antibiotics including iturins. Iturins help B. subtilis bacteria out-compete other microorganisms by either killing them or reducing their growth rate (CPL 2002). Iturins can also have direct fungicidal activity on pathogens. B. subtilis products are made for many uses. For plant disease control, these include foliar application and products applied to the root zone, compost, or seed. When applied directly to seeds, the bacteria colonize the developing root system, competing with disease organisms that attack root systems. B. subtilis inhibits plant pathogen spore germination, disrupts germ tube growth, and interferes with the attachment of the pathogen to the plant. It is also reported to induce systemic acquired resistance (SAR) against bacterial pathogens. Colonies of B. subtilis take up space on the roots, leaving less area or source for occupation by disease pathogens.


Iturins are reportedly active against the fungus Sclerotinia fruticola, which causes rots of harvested stone fruit. B. subtilis has also been tested for control of the pathogenic fungus Verticillium. B. subtilis has been used in conjunction with Streptomyces gramicifaciens for control of root rot in cucumber, corky rot of tomato and carnation wilt. Inoculated with B. subtilis also shows increased resistance to fungal diseases. It is also claimed to suppress diseases caused by Fusarium spp, Rhizoctonia spp and downey mildew in grapes, and powdery mildew on greenhouse tomatoes.

1. The Efficiency of Bacillus subtilis, from Fermentation of Soybean Meal and Molasses, to Inhibit Anthracnose Disease in Oil Palm Plants “Click link

2.Serenade fungicide cotains B. subtilis.

3. Method for producing bactericide or soil conditioner containing bacillus subtilisclick link

Effective viable count :
200 x 108 cfu/gm

20~30gm / 20 Liters
( Activate the mixture with same amount of molasses > 2 hours for better result )

Standard packing :
MYR40/500gm,  MYR70/Kg

Precautions :
Avoid mixed with any fungicide.
Avoid high temperature, moisture and direct sunlight in storage


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