Artificial inoculation

人工處理植入催化劑

Artificial inoculation technique already been developed and is found to be most effective and reliable method for enhancement of agar production. Works on commercialization are in progress and expected to make available for general use in the field.

The Agarwood created by artificial inducement is called Cultivated Agarwood or Induced Agarwood. Artificial inducement technique (or Agarwood inducement) consists of two actions: to wound the tree and to pour stimulative agent into this injury, this stimulative agent hereinafter referred to as inoculant. Quantity and quality of cultivated agarwood surrounding the injury depends on the composition of inoculant and the method of transfusing inoculant into the tree.

Nowadays, there are about several tens of inoculants whose composition is kept secret. But in general the main components of inoculant are:

  • Some fungi and microfungi, for example: Fusarium oxysporum Schlecht., Cladosporium spp, Cercosporella spp, Aspergillus Phoenicis (Corda) Thom & Currie, Cytosphaera Manganiferae Died, Melanotus flavolivens (BerPlant hormonesk. & M.A. Curtis) Singer, Penicillium Citrinum Thom, Chaetonium globosum Kunze, Phaeoacremonium parasitica,…in which Fusarium, Cladosporium and Cercosporella are three of 17 fungi that were found in 9 natural agarwood samples collected from the forest in Thailand, Fusarium and Chaetonium are two fungi found in natural agarwood pieces collected from India.
  • Some phytochemicals, phytohormones namely: salicylic acid, ethephon, methyl jasmonate, chitosan, sodium bisulfite,..
  • Some bacteria, yeast extracts, plant nutrient media and plant regulators

Two main methods Agarwoodof transfusing inoculant into Aquilaria tree:

Dispersing wounding by mechanical injury: to drill several tens (20-100) of hole into which the inoculant is poured. In this case induced agarwood is formed around these spot injury sites. The resulting agarwood from the localized formation is usually in the form of thicker wood chips.

Internal wounding: to transfuse inoculant directly into tAgarwoodhe xylem vessels of tree by using transfusion set, in this case only few holes are needed. If the internal wounding technique to be applied then induced agarwood is formed in whole tree along the xylem, from root/base up to the top of the trunk in a systemic manner, but relatively thinner formation of resinous wood.

Lately, inoculant operators have adopted to Dispersing wounding, leaving the Internal wounding for few reasons after gaining numerous field experiences … wood rotting is the main drawback.

gaharu

wood rotting by internal wounding

While internal wounding is preferred by certain people, it can be further improved with “Pressurized method”. The air pressure can be portably pumped range 0.5 ~ 1.5 bar, hence minimize local concentration which resulting in tissue rotten. For cost effective, unit can be connected to multiple points, and reusable in next application. This invention is simple, inoculation process completed at faster time, no ingress of rain water even the injection holes are not sealed. Click “Mini pump“.

Pressurized method

Dispersing wounding, mechanically wounded with nails, traditional way in Bangladesh

Dispersing wounding, tree drilling and chemical injection.DSC_4217-Edits800

This effort involves drilling of trees and keeping the wound open by placing a small piece of plastic pipe in those holes followed by a chemical injection to stimulate tree defense mechanism that produces resin. The first project of this effort was initiated in Vietnam under a supervision from Prof. Robert Blanchette, a wood pathologist from the University of Minnesota who together with local farmers and Buddhist monks. They developed experimental plots to stimulate the production of agarwood and after years of trials, this treatment yielded agarwood. Dr. Blanchette stated that this artificial induction could yield agarwood ten times faster than natural formation (WWW page: http://forestpathology.coafes.umn.edu). This finding has gained appreciation and has been considered as one of the most successful finding (Persoon, 2007). This treatment causes tree to respond in two defense mechanisms, physical and chemical. The first is phloem cells form callus and the second, should callus formation is prevented, the tree produces resin.

Dispersing wounding with 10~20ml liquid inoculant, some inoculant service providers utilize upto 100ml per treated hole.

Dispersing wounding 1Dispersing wounding 2Dispersing wounding 3Dispersing wounding 4

Dispersing wounding, inoculant-soaked bamboo stick, Thailand
CIMG0467

Dispersing wounding, inoculation of fungal inoculum6158739645_8725dbb2b6_b

The formation of agarwood is a result of plant defense mechanism towards fungal attacks by producing resinous compounds as secondary metabolite. In their natural habitat, the process of resin accumulation as the result of tree-pathogen battle may take many years and the longer the process takes place the more expensive and highly valuable the resin is. Many scientists have been passionately trying to understand the cascade process of this tree-fungi interaction in producing gaharu. Isolation of various fungi from infected trees have been widely reported.

Surface Inducement Technique ( Agar-SIT )… inoculant is designed to brush on the tree trunk. This technique allows easy agarwood collection, a considerably high yield and good resinous of agarwood could be harvested, along with the sustainable agarwood formation from the same tree. Specifically, this technique is aimed for oil production. Click “Agar-SIT-105” for detail …

trunk surface inoculantTrunk stree

Dispersing wounding, non-destrustive harvest, by Pheeraphan Thailand

Thai dispersing 1

gaharu

agarwoodagarwood

Thai dispersing 2Thai dispersing 3

Thai dispersing 4

The infected tissue is stripped by chisel

Internal wounding, Single unit, 300ml inoculant per tree,  by PENGHARUM.

Internal wounding, Multiple units, by Gaharu Biotech,Pahang.

Picture2

Gravitically flow.

wounding

Application of Chemjet, pressure exerted 0.75bar. Test plot to verify strength of inoculant

Inoculant on SinensisClick Here

Artificial fire method, China人工火烧法It is to burn the live agarwood tree with a large fire, forcing the agarwood tree to be injured. It looks very cruel, but it does not let the agarwood tree die, so the agarwood tree will mobilize the resin secreted by the immune system to heal the fire, and eventually form agarwood. Select the older tree, peel off the bark from the tree, leave the top leaves, and then use  gas torch to burn the whole tree and burn it black. This method is derived from the natural fire or the process of lightning strikes. The agarwood produced in this way is mostly thin, it is used a lot to produce incense.

inoculationIn China, newer species of Kynam has been successfully grafted, this species requires mechanical stress without any injection of liquid inoculant to produce resinous agarwood. Application of soldering device is sufficient for the job. However, the seedlings demand extremely premium price for the moment, click “Kynam

kynam23.jpgkynam24

 

 

 

 

 

 

 

Planting money (Agarwood) trees doesn’t come without its challenges. One of these challenges is the inoculation. The Aquilaria tree has to be induced with a infection to induce production of agarwood (the resin), otherwise the tree would be quite worthless. Not all Aquilaria trees produce agarwood, they have to be infected by a certain kind of stress before they start “fighting back” with resin production to counter the infection. And even so, there is no set volume in the resin production (meaning each tree will produce its own amount).

Newbies blinded by the agarwood get-rich-quick scheme are often scammed through these so-called high quality inoculant which “guarantees” high rates of return. Till today, techniques are still being improved to artificially induce optimum agarwood production. There are “many roads that lead to Rome”, with trial and error being part of the journey but there are no guarantees.

The inoculation cost for a 5-8 years old tree in Malaysia ranges 30 ~ 50USD /tree inclusive labour (Time of writing … Dec 2018). Being a layman grower, this can be an astonishing figure if you have 1000 trees to inoculate.

Try this idea …. success

This product is available in local market (Malaysia), but it is sold under different trade name, it is mainly used to treat Durian disease, such as Canker. Phosphite is used as a biodegradable fungicide to protect plants against Phytophthora dieback. Phosphite (PO3) molecules, which move systemically via the phloem and xylem to the leaves and roots. This means that an entire plant receives many benefits of the phosphite molecule. With phosphite, a plant’s natural defense mechanisms are heightened … Phosphite encourages the nucleus of a plant cell to produce defensive molecules such as phytoalexin, which defense the tree from attacks by external intruders … you may try this at higher dosage to blend your inoculant recipe.

Are you running low on budget and NOT resourceful to do the trial … check for this “Option

Do not judge the book by its cover…. whether the inoculant is purchased from 3rd party or home-made, judging on the outlook of cultivated wood alone is insufficient without performing the oil analysis. To analysis the content of VOCs in “cultivated agarwood” we have to extract its essential oil and make GC-MS analysis on this essential oil for seeking specific compounds of natural agarwood. Click “Oudh quality” for detail … and NEVER proceed with mass inoculation without understanding the content of GC-MS report.

Whatever informations posted here is FREE to share, nevertheless if you required Private site“for inoculant guidance, you may whatsapp me @

WhatsApp  : Tee … 00 – 6 – 012 – 323 1161

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169 Responses to Artificial inoculation

  1. ripal chakma says:

    dear sir,
    Your post is really helpfull and very informative.
    from bangladesh, how i can get ICU inoculant and how much the price?
    Thanks.

    • tshinkou says:

      I suggest to use “Bamboo stick”, many types of inoculant available in the market, you really have to do your own survey. I have seen Bamboo stick result, it is proven.

      • Ripal Chakma says:

        dear tshinkou, thanks a lot for giving me your valuable advice. but i m from khagra chari hill district, which is very remote area of bangladesh. i really don’t have any idea of “Bamboo stick”.
        can i make ” Bamboo stick” by using local bamboo? if i can’t,then how i can get this n
        how much?
        thanks again.

  2. tshinkou says:

    These Bamboo sticks have been soak with unique inoculant a day or 2 prior to delivery by manufacturer. ICU method will cause rotten in the tissue, you still get the gaharu but harvest is reduced, The shape of gaharu is hollow in the middle. Pls click “https://gaharujinkou.wordpress.com/product/bamboo-stick-inducer/” for Bamboo stick detail.

  3. NUr Sakinah Ismail says:

    how long does it takes to see the result for each technique?

  4. tshinkou says:

    Most of the techniques recommend a minimum 2 years for better result, the idea is, longer waiting time better the quality.

  5. Kenneth Wu says:

    Hi Tshinkou,

    Very informative website but I can’t find the name of Bamboo Stick manufacturer in the products catagory. Please let me know the name and the address of the manufacturer. Thanks.

    Kenneth

  6. tshinkou says:

    The invention of such product is definately not from the big corporate. Many Agarwood growers claim thier products are the best, seriously i have tried few inoculants, and awaiting for the result.
    However i have seen the Bamboo stick “Cultivated Agarwood” in mass production, that why i am sharing my thought here that Bamboo stick might be one of the priority in the consideration list.
    Try a pack or 2, result will tell which is the best, happy surfing.

  7. Francis says:

    Thanks, your website is very informative. May i know the supplier of Bamboo Stick inducer in Malaysia.

    • Johny thomas says:

      Dear Sir,
      I am interested in bamboo Stick inducer.
      I have only one tree of 13 years old. Recently only I came to know about the value of it.
      How many sticks I have to use?
      Can you reply with details

  8. CJ says:

    can i know what are the inoculant use for the agarwood
    thanks….

  9. tshinkou says:

    Dozen of Inoculant available in the market, they are mainly home-made products. Unable to tell what are they. You can invent one of them and name it with your commercial name. Seriously, everyone claim their products are the best.

  10. Htun Wai says:

    Thanks, your website is very informative.

    • tshinkou says:

      Many veteran players refrain themselves from sharing their knowledge and hard-earned experiences, and hardly you will bounce to them in the net.

    • Htun Wai says:

      I would like to know agarwood inoculation with EM 1 method is a best one.

      • tshinkou says:

        Effective Microorganisms or EM is a mixed culture of beneficial microorganisms that can be applied as an inoculant to increase the microbial diversity of soils. This in turn, can improve soil quality and health, which enhances the growth, yield, and quality of crops.

        EM, my recommendation here is to promote plant growth. I don’t mean to inoculate the tree with EM, but you may try EM1 coupled with your own recipe.

  11. Golap Gogoi says:

    Hi, Very informative page, Recently I read about Agar WIT Technique from http://www.mdpi.com/1420-3049/18/3/3086/pdf‎ . m trying this type of method in my place Assam, India and waiting for the result.

    • tshinkou says:

      Thanks for shaing. Ensure you are confident with the products chosen.

    • Sham says:

      Hi Golap,

      I’ve also read about the Agar wit technique.. Can I know, where I can purchase the Agar witt? By the way, Im from Brunei. Thanks.

    • Johny thomas says:

      Dear Golap
      I am from Kerala. I have an Agar tree of 13 years old. Can you give more details about Agar wit method?
      Where the chemicals are available?
      Can you please give your contact details?
      My No.is 00968 99053791( in Oman)
      sunjohny@hotmail.com

      Pleasereply

    • Patrick Tan says:

      Hi Gogoi, I hv also read about it and wonder how is yr progress of using It. I am keen to acquire some for testing & wonder what is the cost for per tree and how much is required for each tree and for how long? I am also wondering what tree age or size would be idea for agarwit testing?

    • Nilesh Patel says:

      Hello Sir… I am Nilesh from Gujarat, India, Plz give me details about agar inoculation mathod, I will pay you, My email id is omshantinilesh@gmail.com and my mobile no. is +919426962861 Thank you…..

    • kaushik says:

      Hi Golap,
      Recently came to know about agar wit, would also like to try it. Have you got the result. I am also from assam where from to get technology and price thereof.

    • kaushik says:

      Hi Golap,
      Recently came to know about agar wit, would also like to try it. Have you got the result. I am also from assam where from to get technology and price thereof.
      Kaushik.
      Mobile 9401532257

    • prasanth says:

      Hi Golapgi,
      Im from kerala ,i would like to know where we can get the fungus for doing the artificial inoculation,can you please give your contact number,+919747332551

  12. Mr. Zul says:

    Hi Mr. Tee,
    That mean inducer better than inoculant technique? is it bamboo stick can be used for any species of aquilaria?

    • tshinkou says:

      Each method has its unique Pros and cons, but inducer way is the improvement method from the inoculant after years of experience of the inventor. Yes, regardless of the species.

  13. Azwaj says:

    Thanks a lot for your very informative page.
    We have gone through your impressive page while browsing latest inoculation techniques for our agar plants which have become matured. You are, perhaps, aware of Bangladesh and we have about 60,000 agar plants. We need to start inoculation in coming monsoon. In Bangladesh, nailing is the only traditional way of doing this. Although CA kits have been applied in earlier years, it could not bring any success rather damaged most of the inoculated plants.
    Based on your experience, modern technology & all the other aspects, it would be great if we can get your kind advice and assistance regarding Agar Inoculation Techniques that can be followed by us in Bangladesh in the upcoming days.

    Would like to receive your reply at your earliest convenience.
    Regards,

  14. Aruna says:

    Thank you for your great information’s.It’s very helpful to me I wanna know what are the chemicals we should use for the artificial inoculation in agar wood?

  15. S.Wijesiri says:

    I am a project writer and presently writing a book beneficial rural Sri Lanka .Your approval sought to COPY the pictures in the page https://gaharujinkou.wordpress.com/cultivated-agarwood/inoculation-method/.Early attention is highly appreciated.

  16. S.Wijesiri says:

    Walla gyrinops one of an agarwood species available in Sri Lanka which is under threat of extinction.Low income rural villagers involvement is very high in this process. Improvement on knowledge on agrwood , making income sources reduce this devastation.My book target these goals. Your approval sought to copy pictures available in https://gaharujinkou.wordpress.com/cultivated-agarwood/inoculation-method/
    Early response is highly appreciated.

  17. SHINODA says:

    WHAT CHEMICAL IS USED FOR THIS INOCULATION??AND WHAT ARE THE RIGHT COMPOUND FOR INOCULATION OF GAHARU WOOD

  18. abdullah ahmed says:

    hello mr
    tshinkou… i am from india and also a student of microbiology. as i am working on my summer project on artificial inoculation of agarwood.. so i want to talk to you in mail. please if u mail me… i wil be waiting for your swift reply sir
    thank you

  19. kyaw Myo Aung says:

    I have about 7000 trees (Supintegra ) 4 years old. May I know the effective CA kit for it. I plant in Myanmar(Burma). I would like to buy CA kit. Could me advice pls. thanks

  20. dev says:

    Pls send me you are email..

    • dev says:

      Hi….dear sir….. i am from sri lanka….i have to agarwood tree….I need to agarwood cultivated how about if I wanna buy your bamboo stick and chemical..are u can dilevery to sri lanka ???????? Thank u

  21. David says:

    Hi

    can you please inform if you tried the Agar Wit Method and if successful where we may purchase the kits

  22. Lalremi Zote says:

    I have more than 1,00,000 agarwood trees, which are around 30 years now, some are above 50 years old. I want to inoculate them. But i don’t know where to purchase or use them. Please give me some advice. I would be very happy to get information about it.

  23. Moses Anawe says:

    Please I really need to get the ICU Inoculant so how much does it cost?

  24. Milon Chowdhury says:

    I am from Bangladesh. I need some information about nailing method such as nailing period, time required and result. Please reply as soon as possible.

  25. Raheez says:

    I want fungus imacula treatment for agarwood.t want wholesaleprices.south India most agarwood plant.I can get your fungus. Treatmenttra

  26. Johny Thomas says:

    I am interested to know more about agar wit inoculation method. Please advice the details of method.
    I am from Kerala and I have 10 year old tree.
    Please reply. My contact no.is 00968 9905 3791 oman
    In India 094970 77555

  27. tshinkou says:

    Bamboo sticks method is an improvement version to Liquid Inoculation when the inventor founf much rotten occured to the tree which further reduced to yeild, the inventor finally adjusted the dosage to suit field application. This does not indicate that liquid inoculation is too bad to be used, it is up to each individual to judge by himself which method suits him.

  28. budi suprayitno says:

    Informatife …makes more information..

  29. liyanage says:

    In Sri Lanka now interest for cultivation of agarwood has initiated. Information related to cultivation,different varieties on seeds and plants suitable for local conditions,technologies,places available,agar oil processing,relevant machinery,all the information now included in “Agarwood Ayojana Awastha saha Wallapatta ” available at Sarasavi Bookshop,Nugegoda and Gunasena,Pettah.

  30. wasantha says:

    I’m from Sri Lankan. I like to know about this. What are the ingredients wetter? Please help me. thank you.

    • Janaka Opallage says:

      Dear Wasantha,

      Pls contact me on 0094 776029162, so I will give you details on buying Agar plants, growing them, inoculating and harvesting with a secured buy back.

      Look forward to your call
      Cheers!
      Janaka

      • Dear Janaka, sorry for interference , I am from Fiji island , some one has starting planting Agarwood here. and its grow very very well because of tropical and very good mix of weather here.
        But after going through your massage to Mr Wasantha from Shirilanka.

        Makes me very interested if you can provide me with the same condition or your buy back deal .
        Please can you reply back to my email address below .
        iowane2014@gmail.com
        Phn +6798668746
        Fiji .

  31. dulaldey says:

    Very very good

  32. tariqul islam says:

    dear sir.
    i am from Bangladesh. i want to know how ccan i make a best quality vaksin for agartrees.

  33. tariqul islam says:

    sir.
    i shall be highly glad if you kindly give me a best quality agartrees vaksin manufacturing formula and using procedure .
    thanks .

  34. S.WIJESIRI says:

    In Sri Lanka most of the explanations and guidance on agarwood cultivation and technical aspects are available in the book”Agarwod Ayojana awastha saha Wallaptta ‘ written by S.Wijesiri,Project Consultant available at Sarasavi Bookshop -Nugegada,Godage-Maradana and Gunasena -Pettah.This has been appraised by almost all users.

  35. Subrata Sarkar says:

    Hello sir
    I m from Tripura, north east India. I have two agarwood trees. The age of both the trees are near about 20-25yrs. The size of these trees are also big with noticeable diameter. I want to use Bamboo sticks for creating agar on the trees. How many months or year will require for getting the results.I want to purchase the bamboo sticks. How many sticks will require for this operation and from which place I can find this sticks in India or how can I purchase from other country. Please reply me sir.
    Thank u

  36. galley dorjee says:

    i am from bhutan and would like to initiate the plantation of agarwood, and i got few samplings , so i would be indebted if sir could kindly provide me with an information about how to induce it and what kind of inducement we have to provide for the successful treatment and better result.. name some chemical to inoculate the plant,,i heard little about CA kit induced in the agarwood, so how effective it is? and how many years it takes to give agar ? thank you ,, hope ull get good information about it,, thank you sir,

  37. galley dorjee says:

    i am from bhutan and would like to initiate the plantation of agarwood, and i got few samplings , so i would be indebted if sir could kindly provide me with an information about how to induce it and what kind of inducement we have to provide for the successful treatment and better result.. name some chemical to inoculate the plant,,i heard little about CA kit induced in the agarwood, so how effective it is? and how many years it takes to give agar ? thank you ,, hope ull give a good information about it,, thank you sir,plex email me

    • tshinkou says:

      I am afraid i am not the one expert to comment, since cultivated wood demand is still limited as of today. If u have potential Agarwood products development, u can proceed with commercial planting. Whatsapp me at 00 – 6 – 012-323 1161.

  38. Yashawant says:

    I am from Maharashtra State in India and interested in commercial plantation of Agarwood trees. Can any one help me getting the detail of any company which can provide me good quality and authentic saplings and also provide technical support including buy back etc?

  39. George says:

    Hello Tshinkou, How are the prices iike for artificially induced agarwood vs naturally produced agarwood (end product i.e. wood chips), also in quality will the artifically induced come close to the natural one?

    • tshinkou says:

      As long as natural wood supply is avialable and buyers are able to pay….. cultivated wood may need some time to find its room in the market …most probably next 10 years, when the natural wood stock in the middle east warehouse is depleting.

  40. Abdullah Al Mamun says:

    Can anyone inform which university of department is best for higher study and research on Agar wood

  41. Paul Murimattathil Paulose says:

    Hello Tshinkou,
    I have four trees in my court yard and completed about 20 years and out of that two plants are some what grown up and bottom girth may be 30-36 inches and height more than 12 meters. Can you please suggest somebody in Kerala for the inoculation for the said trees on guarantee basis. Kindly send back a feedback as well.

  42. tshinkou says:

    pls chat me thro whatsapp ….

  43. Gary Robert says:

    Helo sir,
    I’m from malaysia, sabah..i have 5 hectar of vacant land and was thinking to plant with agar wood..
    Your post is really helpful and adding my confindence in investing this planting.
    Can you share some information when do i can start using this method and how much does it cost for 1 tree?..
    How can i get this “bamboo stick” ?

  44. Ahmed says:

    Anyone selling pure oud oil in Bangladesh, kindly contact me via mail: iatrading.bd@gmail.com

  45. Omar says:

    We have quite a few agar trees in our tea estate in Bangladesh and planing to go for inoculation. Just wanted to know can CAkits give good results?

    Thanks

  46. BhimRaj Thapa says:

    Do you supply CA Kit?
    How much price?

  47. BhimRaj Thapa says:

    Sir,
    I am in corresponding with a supplier in of CAKit Malaysia…Company name is Tanali Esteem, Selangor..Puchong. Big money at stake here. Hard saved money. Kindly help me find out if the office exists.

  48. abdulsiyad says:

    my name is abdulsiyad i am from kerala ( india ) i have some agarwoods tree and my location have morethan lakh trees i thing start the business here please help and infermation thank you please send me replay assalamualaikkum

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